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Image Search Results
Journal: Biology of the Cell
Article Title: Generation of Intestinal and Colonic Organoids Derived From Human Pluripotent Stem Cells
doi: 10.1111/boc.70044
Figure Lengend Snippet: Overview of the protocol steps. The complete directed differentiation process lasts for 37 days, including 28 days of culture in Matrigel. Two days after seeding the hPSCs, the first step involves adding Activin A for 3 days to generate definitive endoderm formation, followed by the addition of FGF4 and CHIRON for 4 more days to induce spheroid formation. The resulting spheroids are then embedded in Matrigel and cultured for 28 days, with a passage at day 14 to renew the Matrigel.
Article Snippet:
Techniques: Cell Culture
Journal: Biology of the Cell
Article Title: Generation of Intestinal and Colonic Organoids Derived From Human Pluripotent Stem Cells
doi: 10.1111/boc.70044
Figure Lengend Snippet: Characterization of definitive endoderm induction and spheroids generation. (a) Immunofluorescence images for FOXA2 and SOX17 of definitive endoderm after 3 days of Activin A induction. (b) Immunofluorescence images of hindgut spheroids following 4 days of FGF4 and Chiron induction, characterized by CDX2 (intestinal marker) and E‐cadherin (epithelial marker) expression. Scale bars = 100 µm.
Article Snippet:
Techniques: Immunofluorescence, Marker, Expressing